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. 2018 Oct 1;8(10):2020–2029.

Figure 5.

Figure 5

LINC01296 binds to EZH2 and epigenetic silencing of KLF2 expression in ESCC cells. A, B. RIP experiments with anti-EZH2 and anti-H3k27me3 antibodies were performed in EC106 and EC9706 cells. QRT-PCR was used to analyze the expression of LINC01296 in the co-precipitated RNA. The fold enrichment of LINC01296 is relative to the matched IgG control. C. QRT-PCR was used to analyze mRNA expression of KLF2 following treatment with si-NC or si-EZH2 in EC106 and EC9706 cells. D, E. Western blot was used to analyze the expression of KLF2 protein following treatment with si-NC or si-EZH2 in EC106 and EC9706 cells. *P<0.05. F, G. ChIP-qRT-PCR of EZH2 occupancy and H3K27me3 binding in the KLF2 promoters in EC106 and EC9706 cells following treatment with si-NC or si-LINC01296-1 (S1). IgG as a negative control. Error bars indicate the mean ± S.D. *P<0.05.