Figure 3.

Genome-wide identification of transcriptional targets for the BRMS1/LSD1 complex. A. MCF-7 cells were transfected with shSCR, shBRMS1, and shLSD1 followed by RNA extraction and deep sequencing. Three independent samples were used in the RNA-seq analysis. mRNA expression data were clustered using Cluster 3.0 software. B. Intersection of BRMS1- and LSD1-regulated transcriptomes reveal 352 co-regulated genes with a fold change ≥ 2. C. GO analysis of the co-target genes of BRMS1 and LSD1 were performed to further explore the function of BRMS1/LSD1 complex. D. GSEA results showing enrichment gene signatures related to EMT in MCF-7 cells infected with shSCR or shBRMS1. E. MCF-7 cells were transfected with the indicated shRNAs, followed by RNA extraction and qRT-PCR analysis of the expression of indicated genes. The mRNA levels were normalized to those of GADPH. Since the shBRMS1 #1 and shLSD1 #1 are more effective, we chose them for the following experiments. *P < 0.05 and **P < 0.01 (two-tailed t-test).