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. 2018 Oct 1;8(10):2005–2019.

Figure 3.

Figure 3

Immunocytochemical analysis of intracellular localization of FITC labeled aspirin (ASP) c-SLN in (A) Panc-1 cells, and (B) MIA PaCa-2 cells at 30 min, 6 h, and 24 h. DAPI (blue color) shows nuclear staining; FITC labeled ASP c-SLN (green color) shows uptake of ASP c-SLN; and LysoID (marker for lysosome labeling, red color) shows lysosomes in the cytoplasm; Co-localization (yellow color) of red fluorescence from LysoID with green fluorescence from c-SLNs suggests that ASP c-SLNs accumulate in lysosomes (Scale bar - 100 px (1.17 µm/px)). [http://www.techinst.com/userfiles/files/How%20To%20Calibrate%200020.pdf].