Fig. 3.

N-glycome profiles. The chemical nature of individual glycans was established by a combination of MS/MS, GC/MS, and NMR, in comparison with previous work^28,29 and reported in Supplementary Figure 1 and Supplementary Data 1. a Representative MALDI-TOF MS spectra of permethylated NGs isolated from brain. All MS spectra are provided in Supplementary Figure 1. b Tissues-specific distribution and relative quantification of neutral and (c) sialylated (right panel) NGs according to following glycan epitopes: core fucosylation, (Galβ1-4)_0-1Galβ1-4(Fucα1-3)GlcNAcβ, Galβ1-4Galβ1-4GlcNAcβ for neutral and nature of substituting sialic acids (Neu5Ac, Neu5Gc, Kdn), Siaα2-3Galβ1-4(Fucα1-3)_0-1GlcNAcβ, Galβ1-4(Siaα2-3)Galβ1,4(Fucα1-3)_0-1GlcNAcβ, and Siaα2-6GalNAcβ1,3/4GalNAcβ. All Sia residues (Neu5Ac, Neu5Gc, Kdn) linked to Gal were considered as α2,3 linked although minor α2,6-Gal may co-exist as described in the text. Relative quantifications of individual compounds were extracted from the dataset presented in Supplementary Figure 3