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. 2018 Nov 7;8:16499. doi: 10.1038/s41598-018-34738-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Plots of combined (A) embryo mortality percent, (B) mean embryo survival time, (C) embryo hatching percent, (D) mean time to hatch, (E) fry survival percent, and (F) mean fry survival time, of channel catfish (Ictalurus punctatus) embryos microinjected at the one-cell stage with different dosages of sgRNAs/Cas9 protein targeting the toll/interleukin 1 receptor domain-containing adapter molecule (TICAM 1) and rhamnose binding lectin (RBL) genes. Two controls were used for each gene; injected control embryos (iCTRL) were full-siblings to the treatment groups and were injected with the same solution and volumes, but without sgRNA or Cas9 protein. The second control was not injected (nCTRL). Guide RNA/Cas9 protein dosages included low dosage: 2.5 ng gRNA/7.5 ng Cas9 protein, medium dosage: 5 ng gRNA/15 ng Cas9 protein and high dosage: 7.5 ng gRNA/22.5 ng Cas9 protein. Hatch rate was calculated as the number of embryos that hatched at a given time-point (day post-fertilization, dpf) compared to the total number of embryos that hatched (see Table 3).