Table 1.

Total number analyzed, number of mutated individuals and mean mutation rate (% of individuals detected with mutations) of channel catfish (Ictalurus punctatus) dead embryos and embryos surviving to the fingerling stage (four months) that were microinjected at the one-cell stage with three dosages of gRNA/Cas9 protein targeting toll/interleukin 1 receptor domain-containing adapter molecule (TICAM 1) gene and rhamnose binding lectin (RBL) gene.

Treatment	Dead embryos			Surviving fingerlings
	Total analyzed	Mutated	Mean mutation % ± SEM	Total analyzed	Mutated	Mean mutation % ± SEM
TICAM 1*
Low	22	15	68.2 ± 5.51	50	30	60.0 ± 1.12
Medium	19	16	84.2 ± 11.81	13	9	69.2 ± 28.57
High	25	22	88.0 ± 7.39	16	12	75.0 ± 13.33
RBL**
Low	26	23	88.5 ± 7.22	33	26	78.8 ± 4.84
Medium	26	24	92.3 ± 5.82	12	10	83.3 ± 2.86
High	28	26	92.9 ± 4.17	14	13	92.9 ± 4.17
TICAM 1 and RBL combined***
Low	48	38	79.2 ± 5.35	83	56	67.5 ± 4.96
Medium	45	40	88.9 ± 6.17	25	19	76.0 ± 12.18
High	53	48	90.6 ± 4.08	30	25	83.3 ± 7.76

The three gRNA/Cas9 protein dosages were: low dosage: 2.5 ng gRNA/7.5 ng Cas9, medium dosage: 5 ng gRNA/15 ng Cas9 and high dosage: 7.5 ng gRNA/22.5 ng Cas9.

^*Positive correlation between gRNA/Cas9 dosage and mutation rate (p = 0.084).

^**Positive correlation between gRNA/Cas9 dosage and mutation rate (p = 0.025).

^***Positive correlation between gRNA/Cas9 dosage and mutation rate (p = 0.013).