Table 2.

Number and percent of channel catfish (Ictalurus punctatus) 4-month-old fingerlings with congenital anomalies due to microinjection of three different dosages of gRNAs/Cas9 protein targeting toll/interleukin 1 receptor domain-containing adapter molecule (TICAM 1) gene and rhamnose binding lectin (RBL) gene.

Gene	Dosage	Number and percent of fingerlings with anomaly*				Total number
		Absence of eye development	Absence of barbel	Deformed head	Spinal deformity
TICAM1	Low	0 (0%)	0 (0%)	0 (0%)	0 (0%)	50
	Medium	0 (0%)	0 (0%)	2 (15.4%)	0 (0%)	13
	High	0 (0%)	1 (6.3%)	3 (18.8%) (two were mutated, one was not)	0 (0%)	16
RBL	Low	2 (7.7%)	0 (0%)	1 (3.8%)	1 (3.8%)	26
	Medium	1 (10%)	0 (0%)	0 (0%)	0 (0%)	10
	High	1 (7.7%)	0 (0%)	0 (0%)	0 (0%)	13

Three dosages for each gene were injected including low dosage: 2.5 ng gRNA/7.5 ng Cas9 protein, medium dosage: 5 ng gRNA/15 ng Cas9 protein and high dosage: 7.5 ng gRNA/22.5 ng Cas9 protein. All injected and non-injected 4-month-old control fry were free of any anomalies (see Fig. 3).

^*All fingerlings with anomalies were mutated except one from the TICAM 1 high dosage that had head deformity but was not mutated.