. 2018 Nov 7;8:16499. doi: 10.1038/s41598-018-34738-4

Table 4.

The genomic target sequences for gRNAs used to target the channel catfish (Ictalurus punctatus) toll/interleukin 1 receptor domain-containing adapter molecule (TICAM 1) and rhamnose binding lectin (RBL) genes. Underlined sequences represent the protospacer adjacent motif (PAM). (see Figs 1C, 2C).

TICAM 1	RBL
Target sequence for gRNA (3′→5′)
1. GGAGGTGAAGCACGTCGAGGA	1. GGACTTTGAGTCGGAGAAGTGG
2. GGTGACGGAGATTTCTGTGGCGG	2. GGGGCTGGGTACGAAACCTTGG
3. GGGTTCTCGGGTTTATATCTT	3. GGTGAATGCTGACGTAGTTAC
4. GGTGGACTTCGTAGGAGATGAG	4. GGAGTCAAGAGTCTGGGTCTTG
4. GGTGGACTTCGTAGGAGATGAG	5. GGAGTGACACAGGATGTAGACG