Table 5.

Primers used to amplify a partial sequence of channel catfish (Ictalurus punctatus) toll/interleukin 1 receptor domain-containing adapter molecule (TICAM 1) gene and rhamnose binding lectin (RBL) gene flanking the target sites for guide RNAs.

Primer name	Strand	Sequence 5′-3′	Annealing temperature	Product length (bp)
T1F	Forward	GCTGCTGAATGTCTGATTATG	60 °C	750
T1R	Reverse	GTCCTCCACACTCCTGAAG
T2F	Forward	ACTGGTGGACGAGAAGAAG	60 °C	1462
T2R	Reverse	CTGGATGTGGATGTTGGATG
RBL F	Forward	ATCGTGTTGTGATCTGTGAG	57 °C	645
RBL R	Reverse	GCCCTAGCCAATTTGATGTT

Primers T1F and T1R were used to amplify DNA segments containing small indels while primers T2F and T2R were used for the large deletions (see Fig. 1A). Primers RBL F and RBL R were used to amplify a partial DNA sequence of the RBL gene (see Fig. 2A).