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. 2018 Nov 7;8:16492. doi: 10.1038/s41598-018-34835-4

Figure 3.

Figure 3

Time-dependent changes in the phosphorylation status of intracellular signaling molecules. (A) Selectivity of MET receptor activation by aMD5-PEG11. EHMES-1 cells were stimulated with 1 nM HGF or 120 nM aMD5-PEG11 in the absence or presence of the selective MET tyrosine kinase inhibitor, PHA665752 (100 nM) for 10 min. (B) Representative immunoblots for ERK, AKT, PRAS40, STAT3, and CREB. Total ERK, AKT, PRAS40, STAT3, and CREB levels were evaluated to ensure equal loading. (C) Changes in phosphorylation levels of ERK, AKT, PRAS40, STAT3, and CREB. EHMES-1 cells were stimulated with 1 nM HGF or 120 nM aMD5-PEG11 for 0, 3, 10, 30, or 90 min. Phosphorylation levels were expressed as the relative value in percent to the maximal level achieved by aMD5-PEG11. Each value indicates the mean ± SE obtained from independent experiments performed in triplicate.