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. 2018 Nov 7;8:16492. doi: 10.1038/s41598-018-34835-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in intracellular signaling activation by aMD5-PEG11. (A) Arrangement of proteins in the array (left) and representative pictures from array analysis (right). Positions of phosphoproteins showing remarkable increases upon aMD5-PEG11 or HGF stimulation are indicated by red squares. Cells were stimulated with 1 nM HGF or 120 nM aMD5-PEG11 for 10 min. (B) Changes in phosphorylation levels determined by quantitation of the protein phosphorylation array. Phosphoproteins showing remarkable increases upon aMD5-PEG11 or HGF stimulation are indicated by red squares. Each value indicates the mean ± SE obtained from independent experiments performed in triplicate. Representative immunoblots are shown.