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. 2018 Nov 7;8:16506. doi: 10.1038/s41598-018-34873-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Circulatory and lesional unconventional T cell proportions in LCH. (a) Gating strategy for identifying MAIT cells (1^st and 2^nd columns), γδ T cells (3^rd column) and type I NKT cells (4^th column) from circulating lymphocytes in healthy donors (1^st row) and patients with LCH (2^nd row), and from lesional lymphocytes in patients with LCH (3^rd row). (b) Proportions of MAIT cells and Vα7.2 TCR⁺CD161⁻ T cells, γδ T cells and type I NKT cells in total T cells. (c) Proportions of MAIT cells, Vα7.2 TCR⁺CD161⁻ T cells, γδ T cells and type I NKT cells in circulating T cells from patients with LCH (LCH-PB) stratified by active disease (AD) and non-active disease (NAD). Kruskal-Wallis tests with Dunn’s multiple comparisons were conducted for (b) and Mann Whitney tests for (c), error bars indicate median + interquartile range, 10⁻³ on logarithmic scale indicates ‘undetectable’.