Figure 5.

NKT cell subsets in LCH. (a) Gating strategy for identifying type I NKT cell subsets in peripheral blood from healthy donors (HD) and patients with LCH (PB), and in lesions from patients with LCH. (b) Proportions of CD56⁺ cells in type I NKT cells. (c) Proportions of CD4⁺CD8⁻, CD8⁻CD4⁻ and CD8⁺CD4⁻ cells in type I NKT cells. (d) Proportions of CD8⁺CD161⁻, CD8⁺CD161⁺, CD8⁻CD161⁺ and CD8⁻CD161⁻ cells in type I NKT cells. Kruskal-Wallis tests with Dunn’s multiple comparisons were conducted for (b–d) (LCH lesion group excluded due to sample size) except where only two groups were compared (Mann Whitney test), error bars indicate median + interquartile range, 10⁻³ on logarithmic scale indicates ‘undetectable’.