Figure 2.

TM plays a role in eotaxin-2-induced monocytic THP-1 cell apoptosis. THP-1 cells were transfected with TM siRNA or HA-TM FL plasmid for 24 hours followed by 10 ng/mL eotaxin-2 stimulation for 24 hours. The expression of annexin V/PI expression on THP-1 cells was analyzed with fluorescence microscopy (A) and flow cytometry (B and C). Silencer validated siRNA (NC siRNA) and mock plasmid were used to validate the knockdown and overexpression, respectively. Five independent experiments were performed (n=5), and representative immunofluorescence images are shown. Data are presented as the means ± SEM; *P<0.05 was considered significant.