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. 2018 Oct 15;10(10):3133–3149.

Figure 3.

Figure 3

TM modulates BAX and Bcl-2 expression via PI3K/Akt axis signaling in eotaxin-2-stimulated THP-1 cells. THP-1 cells were transfected with TM siRNA or HA-TM FL plasmid followed by eotaxin-2 stimulation for 18 hours (A and B) or 6 hours (C). The expression of Bcl-2, BAX, and p53 and the phosphorylation of Akt on THP-1 cells were analyzed using western blot analysis. (D) THP-1 cells were transfected with HA-TM FL plasmid for 24 hours followed by 10 mM LY294002 for 1 hour then 10 ng/mL eotaxin-2 stimulation for 18 hours (for BAX and Bcl-2) or 6 hours (for p53). BAX, Bcl-2, and p53 were analyzed using western blot analysis. The total-Akt and β-actin were as loading controls. Silencer validated siRNA (NC siRNA) and mock plasmid were used to validate the knockdown and overexpression, respectively. The amount of protein expression was quantified using densitometry and is presented as a bar graph. The data are presented as the mean ± SEM (n=3), and *P<0.05 was considered significant.