Figure 4.

TM modulates mitochondria-mediated apoptosis in eotaxin-2- stimulated THP-1 cells. A and B. THP-1 cells were transfected with TM siRNA or HA-TM FL plasmid followed by eotaxin-2 stimulation for 6 hours. The mitochondria membrane potentials were analyzed using image cytometry. The percentage of JC-1 aggregation in experimental groups relative to the control group represents the decline in the mitochondria membrane potential. The data are presented as the mean ± SEM (n=5), and *P<0.05 was considered significant. C and D. THP-1 cells were transfected with TM siRNA or the HA-TM FL plasmid followed by eotaxin-2 stimulation for 8 hours. The release of cytochrome c and cleaved/activated of caspase 3 and 8 on THP-1 cells were analyzed using western blot analysis. Silencer validated siRNA (NC siRNA) and mock plasmid were used to validate the knockdown and overexpression, respectively. β-actin was employed as a loading control.