Figure 5.

TM may act as a scaffold for cofilin docking, which modulates mitochondria-mediated apoptosis in THP-1 cells. THP-1 cells were transfected with HA-TM FL plasmid for 24 hours followed by 10 ng/mL eotaxin-2 stimulation for 12 hours. A. The total cell lysates were purified and analyzed for the activation (phosphorylation) of cofilin using western blot analysis. b-actin was used as a loading control. B. The mitochondrial fractions were extracted and analyzed for the cofilin using western blot analysis. GRP94 was used as a loading control. C. Total cell lysates of THP-1 cells were immunoprecipitated using a rabbit IgG control, an anti-TM antibody, or anti-cofilin antibody followed by immunoblotting with antibodies against TM, or cofilin, as indicated. Ten-percent inputs were used to identify the specificities of the antibodies.