Figure 5.

The osteogenic induction of BMSCs. BMSCs were cultured in osteogenic induction medium up to 14 days. Alkaline phosphatase (ALP) staining (A) and semi-quantitative analysis of ALP (C) at day 0, day 7 and day 14 showed increased ALP activity as in-vitro osteogenic induction time extended. Alizarin red staining (ARS) (B) at day 0, day 7 and day 14 revealed that extracellular matrix calcium deposition accumulated when lengthened osteogenic induction in vitro. Columns with different letters indicate statistical significance.