Figure 5.

Metformin enhanced cisplatin cytotoxicity in ovarian cancer in vivo. Twenty-four nude mice with xenograft tumours were randomly divided into four groups: the control group (PBS, oral gavage and intraperitoneally), metformin group (200 mg/kg, oral gavage), cisplatin group (4 mg/kg, intraperitoneally) and metformin + cisplatin group (200 mg/kg metformin + 4 mg/kg cisplatin). A. Tumour volume was measured and calculated every three days. B. The mice were weighed every three days. C, D. The tumours were resected and weighed after the mice were sacrificed. *, P<0.05, **, P<0.01, and ***, P<0.001.