Figure 3.

Detection of mitophagy-related protein co-localization with mitochondria in myocardium. Representative images (400×) are shown. Nuclei were stained with DAPI (blue). COX IV which is located in mitochondrial inner membrane was dyed in green while Parkin, p62 and LC3 were dyed in red. The co-located position turned yellow after being merged. (A) parkin co-located with COX IV, (B) p62 co-located with COX IV, (C) LC3 co-located with COX IV (D), quantitative analysis of the amount of co-localization presented by Pearson’s correlation. Values are expressed as mean ± SD, n = 9. Versus Sham, *P<0.05 and versus Model, ΔP<0.05, ΔΔP<0.01.