Figure 3.

HMGA2 silencing or inhibition of the ATR/Chk1 signaling pathway repressed epithelial-to-mesenchymal transition in cervical cancer cells, according to the findings of immunofluorescence staining. Notes: Based on the immunofluorescence staining (A, 400 ×, scale bar: 25 μm) and corresponding quantitative analysis (C) in the HeLa cell line, N-cadherin positive expression was decreased/increased, and E-cadherin positive expression was increased/decreased in response to caffeine/BCNU transfection; In the HMGA2-KD-HeLa cell line, immunofluorescence staining (B, 400 ×, scale bar: 25 μm) and corresponding quantitative analysis (D) indicated that N-cadherin positive expression was increased, and E-cadherin positive expression was decreased in response to pcDNA-HMGA2; *, P<0.05 vs. the blank group; red-stained cells are positive cells, and the data were measurement data, expressed by mean ± standard deviation and analyzed by student t test; the experiment was repeated 3 times.