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. 2018 Nov 7;8:16509. doi: 10.1038/s41598-018-34735-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization and preparation of PIZV candidates. (a) Plaque phenotype of ZIKV PRVABC59 P6 sub-isolates, compared to ZIKV PRVABC59 P1 stock. (b) Growth kinetics of ZIKV PRVABC59 P6 sub-isolates. (c) Flowchart of PIZV preparation. (d) Left panel: total protein on SDS-PAGE. Right panel: Immunoblot analysis detecting ZIKV E. Lane 1: recombinant ZIKV sE; lane 2 and 4: P7 virus supernatant; lane 3 and 5: PIZV made from P7 virus. (e) Representative electron micrograph of PIZV-e at 110,000X magnification.