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. 2018 Nov 7;8:16509. doi: 10.1038/s41598-018-34735-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PIZV immunogenicity in CD1 mice. (a) Test groups and schedule of dosing. (b) Serum NAb titers of immunized CD-1 mice as determined by RVP neutralization assay. Solid lines represent the geometric mean titer (GMT) of EC₅₀ for each group. The limit of detection (1.9 log₁₀) is represented by a dashed line. Samples with a titer below the detection limit were included in the GMT calculation. Data were analyzed by one-way ANOVA with Tukey’s multiple comparisons test: **** indicates p < 0.0001.