Fig. 2.

APOL1 RNAs from high-risk alleles activate PKR. a Stable HEK293FT cell lines expressing APOL1 RNA (but not protein) were harvested for detection of APOL1 and β-actin mRNAs and eIF2α and PKR proteins. Constructs expressing APOL1 risk variant G1 and G2 RNA (without protein) increased phospho-PKR and phospho-eIF2 alpha, whereas the G0 variant had no effect. b APOL1 G1 and G2 RNA promote PKR activation in a time-dependent manner. RNAs (NM_001136540.1, 298–1453) transcribed in vitro using T7 sequence were incubated with PKR and ATP for indicated times. Poly(I:C) served as the positive control for PKR phosphorylation. Vehicle was used for negative control. Western blots targeting phospho-PKR are presented. The quantified intensity of phospho-PKR to each RNAs was added below the gel images