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. 2018 Nov 7;8:16456. doi: 10.1038/s41598-018-34574-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ovariectomy-induced bone loss in vivo. (A) Micro CT analysis of the distal femur from sham and OVX group. Calculations of bone mineral density (BMD), trabecular number (Tb, N), bone surface area/ total value (BS/TV), bone value/total value (BV/TV). (B) H&E staining of distal femoral sections and quantification of trabecular area from sham and OVX group. (***P < 0.001).