Table 2.

Differential metabolites expressed in OVX group.

No.	Name	Formula	m/z	Adduct	Rt(min)	FC(M-C)	P(M-C)	FDR	Pathway
1	Uridinea	C9H12N2O6	243.0607	[M − H]−	3.61	0.49↓	2.83E-08***	4.143E-07	Pyrimidine metabolism
2	Hypoxanthineb	C5H4N4O	137.0458	[M + H]+	3.73	0.78↓	3.13E-04***	6.808E-07	Purine metabolism
3	Ubiquinol 8c	C49H74O4	744.5893	[M + NH4]+	3.93	0.44↓	2.35E-08***	1.785E-05	Respiratory chain
4	Xanthineb	C5H4N4O2	151.0246	[M − H]−	4.58	0.61↓	7.07E-08***	8.762E-07	Purine metabolism
5	Inosineb	C10H12N4O5	267.0722	[M − H]−	5.16	0.60↓	6.77E-03**	1.515E-02	Purine metabolism
6	Cytidinea	C9H13N3O5	242.0768	[M − H]−	6.25	0.31↓	6.09E-09***	1.963E-07	Pyrimidine metabolism
	Cytidinea	C9H13N3O5	266.0750	[M + Na] +	6.27	0.36↓	5.44E-09***	9.595E-05	Pyrimidine metabolism
7	L-Phenylalanineb	C9H11NO2	166.0862	[M + H]+	7.20	0.27↓	5.73E-09***	7.061E-07	Phenylalanine metabolism
8	L-Leucineb	C6H13NO2	132.1019	[M+H]+	7.21	0.29↓	6.00E-09***	9.846E-05	Leucine and isoleucine metabolism
9	L-Carnitinea	C7H15NO3	162.1126	[M + H]+	7.31	0.35↓	5.31E-09***	1.385E-04	Carnitine metabolism
10	L-Prolineb	C5H9NO2	116.0707	[M + H]+	9.35	0.35↓	2.04E-08***	2.927E-03	Arginine and proline metabolism
11	L-Arginineb	C6H14N4O2	175.1191	[M + H]+	16.71	0.41↓	1.40E-05***	1.333E-03	Arginine and proline metabolism

^aMetabolites analyzed based on MS/MS chromatograms.

^bMetabolites validated with standards.

^cMetabolites putatively annotated.

(↓): down-regulated. **p < 0.01, ***p < 0.001 versus Sham.