. 2018 Nov 7;8:16463. doi: 10.1038/s41598-018-34694-z

Table 3.

Expression levels of mexA and mexE in Pseudomonas aeruginosa carrying specific alterations at MexAB-OprM/MexEF-OprN regulators.

Isolates	MexAB-OprM regulators			MexEF-OprN regulators		Transcript Level
Isolates	nalC	nalD	mexR	mexS	mexT	mexA ^a	mexE ^a
1082	G₇₁E, S₂₀₉R	WT	WT	WT	NA	3.58	1.41
1083	WT	WT	WT	WT	WT	0.13	0.71
1084	G₇₁E, A₁₄₅V, S₂₀₉R	NA	WT	NA-c	WT	4.36	0.53
1085	G₇₁E, S₂₀₉R	Δ _nt451–461	WT	NA-c	WT	1.84	0.45
1086	G₇₁E, S₂₀₉R	Δ _nt397–398	V₁₂₆E	WT	WT	5.10	7.92
1087	G₇₁E, A₁₄₅V, S₂₀₉R	WT	V₁₂₆E	NA-c	WT	0.97	1.98
1088	G₇₁E, A₁₄₅V, S₂₀₉R	WT	V₁₂₆E	NA-c	G₁₄₈A, G₂₃₈R, A₂₄₉P	0.80	0.64
1089	G₇₁E, S₂₀₉R	Δ _nt397–398	V₁₂₆E	WT	WT	1.80	1.41
1090	G₇₁E, S₂₀₉R	Δ _nt397–398	V₁₂₆E	WT	WT	1.90	0.83
1091	G₇₁E, S₂₀₉R	WT	WT	NA-c	NA	0.34	0.37
1092	G₇₁E, S₂₀₉R	Q₁₃₄H, Q₁₄₂H, A₁₄₅P, D₁₄₇H, E₁₄₈K, C₁₄₉R, H₁₅₄P, R₁₆₀K, D₁₇₆E, D₁₈₅Y, G₂₀₆S, S₂₀₉I	V₁₂₆E	WT	WT	1.51	0.53
1093	G₇₁E, S₂₀₉R	Δ _nt397–398	V₁₂₆E	WT	WT	3.74	0.83
1094	Δ _{nt 234–243}	WT	WT	NA-b	WT	1.61	1.11
1095	G₇₁E, S₂₀₉R	WT	NA	NA-b	WT	1.04	8.92
1096	G₇₁E, S₂₀₉R	Δ _nt391	V₁₂₆E	G₂₂₄S	WT	1.84	0.13
1097	G₇₁E, S₂₀₉R	Δ _nt391	WT	NA-b	WT	0.38	0.91
1098	G₇₁E, S₂₀₉R, P₂₁₀L	T₁₈₈A	WT	WT	WT	0.85	0.96
1099	G₇₁E, A₁₄₅V, S₂₀₉R	WT	V₁₂₆E	WT	WT	1.26	0.76
1100	G₇₁E, S₂₀₉R	WT	NA	WT	WT	0.80	4.81
1101	WT	WT	WT	NA-c	WT	0.09	1.16

WT: wild type (nalC, nalD, mexR identical to that of PAO1; mexS and mexT identical to those of PA14); NA: No amplification; NA-b: In addition to no amplification of mexS, no amplification of either mexS N- and C-terminal regions (see panel b of Fig. 3); NA-c: In addition to no amplification of mexS, no amplification of the mexS C-terminal region (see panel c of Fig. 3).

Relevant modifications are marked in bold.

^aRelative gene expression was calculated by 2^−ΔΔCT method. The rpsL gene was used as reference, P. aeruginosa PAO1 strain as calibrator in mexA gene (value = 1) and P. aeruginosa PA14 strain was used as calibrator in mexE gene (value = 1). Expression levels increases ranging between 1.5 and 2-fold were considered as borderline, and those increases in the expression levels >2 were classified as overexpression^3,13.