Table 1.
3D MR imaging-based brain volumetric analysis.
| Region | Structure | Sub-structure | Average (mm3) | Change in absolute volume (%) | Groupwise difference uncorrected p value | |
|---|---|---|---|---|---|---|
| WT | Brd1 +/− | |||||
| Cerebral grey | Cerebral Cortex | Frontal Cortex | 46.0 ± 0.9 | 44.7 ± 0.8 | −2.8 | 0.30 |
| Occipital Cortex | 6.5 ± 0.2 | 5.9 ± 0.2 | −9.8 | 0.02 | ||
| Parieto-temporal Cortex | 80.3 ± 2.0 | 78.3 ± 2.2 | −2.5 | 0.51 | ||
| Entorhinal Cortex | 10.1 ± 0.7 | 9.6 ± 0.6 | −5.2 | 0.55 | ||
| 143.0 ± 3.3 | 138.5 ± 3.6 | −3.1 | 0.37 | |||
| Hippocampus | Hippocampus Proper | 24.0 ± 0.4 | 22.7 ± 0.5 | −5.5 | 0.07 | |
| Dentate Gyrus | 4.6 ± 0.3 | 4.1 ± 0.1 | −10.8 | 0.14 | ||
| Stratum Granulosum | 1.1 ± 0.0 | 1.0 ± 0.0 | −6.0 | 0.11 | ||
| 29.7 ± 0.7 | 27.8 ± 0.7 | −6.4 | 0.07 | |||
| Amygdala | 16.3 ± 0.2 | 15.5 ± 0.2 | −5.3 | 0.01 | ||
| Striatum | Caudate Nucleus/Putamen | 20.5 ± 0.5 | 18.4 ± 0.5 | −10.3 | <0.01 | |
| Fundus of Striatum | 0.2 ± 0.0 | 0.2 ± 0.0 | 0.9 | 0.90 | ||
| Nucleus Accumbens | 4.4 ± 0.2 | 4.3 ± 0.1 | −3.6 | 0.45 | ||
| 25.1 ± 0.6 | 22.8 ± 0.5 | −9.1 | 0.01 | |||
| Subiculum (pre-para) | 0.3 ± 0.1 | 0.4 ± 0.1 | 28.9 | 0.38 | ||
| Globus Pallidus | 3.2 ± 0.1 | 3.0 ± 0.1 | −6.9 | 0.03 | ||
| Thalamus | 18.4 ± 0.4 | 17.6 ± 0.5 | −4.1 | 0.27 | ||
| Hypothalamus | 12.1 ± 0.3 | 11.6 ± 0.4 | −3.7 | 0.39 | ||
| Mammillary Bodies | 0.6 ± 0.0 | 0.6 ± 0.1 | 0.1 | 1.00 | ||
| Medial Septum | 1.5 ± 0.1 | 1.4 ± 0.1 | −3.0 | 0.64 | ||
| Lateral Septum | 3.9 ± 0.2 | 3.8 ± 0.2 | −2.5 | 0.76 | ||
| Basal Forebrain | 5.0 ± 0.1 | 4.9 ± 0.2 | −1.8 | 0.68 | ||
| Bed Nucleus of Stria Terminalis | 1.5 ± 0.1 | 1.5 ± 0.1 | 0.2 | 0.98 | ||
| 260.6 ± 5.7 | 249.5 ± 5.7 | −4.3 | 0.19 | |||
| Cerebral white | Anterior Commissure: pars anterior | 1,7 ± 0.1 | 1.5 ± 0.0 | −9.5 | 0.01 | |
| Anterior Commissure: pars posterior | 0,5 ± 0.0 | 0.5 ± 0.0 | −1.9 | 0.80 | ||
| Posterior Commissure | 0.1 ± 0.0 | 0.1 ± 0.0 | −4.5 | 0.43 | ||
| Cerebral Peduncle | 2.6 ± 0.1 | 2.5 ± 0.1 | −4.3 | 0.37 | ||
| Corpus Callosum | 17.1 ± 0.4 | 16.5 ± 0.2 | −3.1 | 0.18 | ||
| Fasciculus Retroflexus | 0.3 ± 0.0 | 0.3 ± 0.0 | 0.8 | 0.87 | ||
| Fimbria | 3.5 ± 0.1 | 3.2 ± 0.1 | −7.4 | 0.12 | ||
| Fornix | 0.8 ± 0.1 | 0.7 ± 0.1 | −2.8 | 0.79 | ||
| Habenular Commissure | 0.0 ± 0.0 | 0.0 ± 0.0 | −11.0 | 0.38 | ||
| Internal Capsule | 3.0 ± 0.1 | 2.8 ± 0.1 | −5.7 | 0.15 | ||
| Mammilothalamic Tract | 0.36 ± 0.0 | 0.3 ± 0.0 | −2.1 | 0.74 | ||
| Optic Tract | 1.9 ± 0.1 | 1.9 ± 0.1 | −0.4 | 0.94 | ||
| Stria Medullaris | 0.8 ± 0.0 | 0.8 ± 0.0 | −2.7 | 0.62 | ||
| Stria Terminalis | 1.0 ± 0.0 | 1.0 ± 0.0 | −5.4 | 0.25 | ||
| 33.4 ± 0.6 | 32.1 ± 0.5 | −4.1 | 0.10 | |||
| Olfactory | Olfactory Bulbs | 29.2 ± 0.5 | 29.4 ± 0.7 | 0.5 | 0.88 | |
| Olfactory Tubercle | 4.1 ± 0.1 | 3.9 ± 0.1 | −3.4 | 0.45 | ||
| Lateral Olfactory Tract | 1.6 ± 0.1 | 1.6 ± 0.1 | −3.3 | 0.48 | ||
| 34.9 ± 0.6 | 34.9 ± 0.9 | −0.1 | 0.96 | |||
| Cerebellum | Arbor Vita of Cerebellum | 9.6 ± 0.3 | 9.7 ± 0.3 | 1.1 | 0.81 | |
| Cerebellar Cortex | 47.8 ± 1.4 | 48.5 ± 1.3 | 1.5 | 0.70 | ||
| Cerebellar Peduncle: Inferior | 1.0 ± 0.1 | 0.9 ± 0.1 | −11.9 | 0.15 | ||
| Cerebellar Peduncle: Middle | 1.5 ± 0.1 | 1.4 ± 0.0 | −5.3 | 0.22 | ||
| Cerebellar Peduncle: Superior | 0.9 ± 0.1 | 0.9 ± 0.1 | −0.1 | 0.99 | ||
| 60.8 ± 1.6 | 61.4 ± 1.6 | 1.1 | 0.78 | |||
| Ventricles | Lateral Ventricle | 4.5 ± 0.2 | 4.0 ± 0.2 | −12.2 | 0.04 | |
| Third Ventricle | 1.6 ± 0.1 | 1.5 ± 0.0 | −6.3 | 0.24 | ||
| Cerebral Aqueduct | 0.7 ± 0.2 | 0.6 ± 0.1 | −17.7 | 0.43 | ||
| Fourth Ventricle | 0.4 ± 0.1 | 0.3 ± 0.1 | −7.1 | 0.74 | ||
| 7.2 ± 0.3 | 6.4 ± 0.3 | −11.2 | 0.06 | |||
| Brainstem | Colliculus: Inferior | 5.7 ± 0.2 | 5.8 ± 0.3 | 2.1 | 0.72 | |
| Colliculus: Superior | 9.3 ± 0.5 | 9.3 ± 0.6 | 0.8 | 0.93 | ||
| Corticospinal Tract | 2.3 ± 0.2 | 3.3 ± 0.6 | 41.9 | 0.14 | ||
| Cuneate Nucleus | 0.2 ± 0.0 | 0.2 ± 0.0 | 1.0 | 0.94 | ||
| Facial Nerve | 0.4 ± 0.1 | 0.3 ± 0.1 | −17.2 | 0.50 | ||
| Inferior Olivary Complex | 0.6 ± 0.1 | 0.7 ± 0.1 | 7.5 | 0.57 | ||
| Medial Lemniscus/Medial Longitudinal Fasciculus | 2.5 ± 0.2 | 2.5 ± 0.1 | 1.3 | 0.87 | ||
| Pontine Nucleus | 0.9 ± 0.0 | 0.9 ± 0.0 | −0.5 | 0.91 | ||
| Superior Olivary Complex | 0.8 ± 0.0 | 0.8 ± 0.0 | −8.7 | 0.18 | ||
| Periaqueductal Grey | 4.2 ± 0.2 | 4.2 ± 0.2 | −0.7 | 0.93 | ||
| Medulla | 25.5 ± 2.5 | 23.4 ± 1.7 | −8.2 | 0.49 | ||
| Midbrain | 14.0 ± 0.4 | 13.8 ± 0.6 | −1.4 | 0.78 | ||
| Pons | 16.8 ± 0.7 | 16.2 ± 0.7 | −3.6 | 0.55 | ||
| Interpedunclar Nucleus | 0.3 ± 0.0 | 0.3 ± 0.0 | −2.1 | 0.76 | ||
| Ventral Tegmental Decussation | 0.1 ± 0.0 | 0.1 ± 0.0 | −1.6 | 0.83 | ||
| 83.4 ± 2.7 | 81.6 ± 2.2 | −2.2 | 0.60 | |||
| Total Brain volume | 464.0 ± 9.6 | 450.4 ± 10.00 | −2.9 | 0.80 | ||
3D MR imaging coupled with deformation-based morphometry analyses of differences in absolute cerebral volumes (whole brain, brain regions, brain structures and sub-structures) of 15 weeks old Brd1+/− mice (n = 9) compared to WT mice (n = 10). The change in volume was calculated as the percentage change between the mean volume of the WT group and the mean volume of the Brd1+/− group. Nominally significant groupwise differences are in bold.