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. 2018 Nov 7;8:16475. doi: 10.1038/s41598-018-34830-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of BMS-345541 in cultured human articular chondrocytes. (a) mRNA levels of MMP13, ADAMTS5, and COL2A1 in human primary articular chondrocytes treated with different concentrations of BMS-345541 with or without 10 ng/ml IL-1β. BMS-345541 and IL-1β were added simultaneously, and the cells were cultured for an additional 24 h. The cells obtained from the three individuals showed similar responses, and a representative result is shown. Symbols represent individual points; long and short bars show the mean and SD of three wells per group, respectively. *P < 0.01, **P < 0.001, ***P < 0.0001 vs IL-1β+/BMS-345541− by the Kruskal–Wallis test and Dunn’s post hoc test for unequal variance. (b) Immunoblot analyses of human primary articular chondrocytes treated simultaneously with different concentrations of BMS-345541 with or without 10 ng/ml IL-1β. (c) Luciferase activities in human articular chondrocytes transfected with the NF-κB reporter vector. BMS-345541 was added 24 h after transfection, and the cells were cultured for an additional 24 h. Symbols represent individual points; long and short bars show the mean and SD of five wells per group, respectively. *P < 0.0001 vs IL-1β+/BMS-345541− by the Kruskal–Wallis test and Dunn’s post hoc test for unequal variance.