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. 2018 Nov 7;9:4657. doi: 10.1038/s41467-018-06565-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The 3α-hydroxycostunolide serves as the intermediate compound in kauniolide formation. a GC-MS chromatogram of kauniolide standard. b GC-MS chromatogram of product of assay with 3α-hydroxycostunolide as substrate fed to yeast microsomes expressing Tp8879: production of kauniolide (nominal mass 230). c Negative control; feeding 3α-hydroxycostunolide to microsomes containing EV (microsomes of yeast with empty vector) does not lead to kauniolide formation