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. 2018 Nov 7;8:16462. doi: 10.1038/s41598-018-34701-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypic effects of maternal RNAi (a) Eclosion rate (±SEM) of female pupae after Dnmt1 RNAi treatment. Three replicates with 240-270 individuals per treatment and block. (b) Larval hatching rates (±SEM) after mothers received Dnmt1 RNAi treatment. Three replicates with 100 individualized eggs per treatment and block. (c) Offspring produced by single mating pairs (n = 26-30) after maternal RNAi treatment (Dnmt1 RNAi construct a or b, RNAi or naïve control). Letters indicate significant differences.