Fig. 2.

Contribution of different lineage cells by hPSCs + BMI1. Sections of E10.5 mouse chimeric embryos were analyzed by immunostaining on selected markers. a Representative images showing the integrated DsRed⁺ human cells coexpress human-specific marker Stem121 in chimeric embryos. Scale bars, 20 µm (left), 50 µm (right). b DsRed⁺ human cells in mouse chimeric embryo were costained with antibodies against Calponin, mesoderm marker, PAX6, ectoderm marker and SOX17, endoderm marker. The insets are the zoom-in pictures of the areas of yellow box. Scale bars, 20 µm (left), 50 µm (right)