Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 7;9:4649. doi: 10.1038/s41467-018-07098-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — hiPSCs + BMI1 integrate into rabbit and pig preimplantation blastocyst. a−d Integration of hiPSCs + BMI1 in rabbit blastocysts. a Representative images of rabbit embryos stained with Annexin V after injection of seven indicated DsRed⁺ cells in blastocysts and 1-day culture in vitro. White arrow, DsRed⁺/Annexin V⁺ cells; Yellow arrow, DsRed⁺/Annexin V⁻ cells. Scale bars, 20 µm. b Images of rabbit blastocysts injected with seven indicated cells after 1-day culture in vitro, scale bars, 50 µm (left), 20 µm (right). c Statistics of the percentage of engrafted DsRed⁺ cells that were Annexin V⁺ in the injected rabbit blastocysts; mean + SEM of 16 (UH10-DsRed) or 14 (UH10-DsRed + BMI1) samples, ***p < 0.001. d Statistics of the DsRed⁺ cells in the injected rabbit blastocysts; mean ± SEM of 16 (UH10-DsRed) or 14 (UH10-DsRed + BMI1) samples, ***p < 0.001. e−h Integration of hiPSCs + BMI1 in pig blastocysts. e Representative images of pig embryos stained with Annexin V after injection of 3–5 indicated DsRed⁺ cells in later morulas or early blastocysts for 1-day culture in vitro. White arrow, DsRed⁺/Annexin V⁺ cells; Yellow arrow, DsRed⁺/Annexin V⁻ cells. Scale bars, 20 µm. f Images of pig blastocysts injected with the indicated cells after in vitro culture. Scale bars, 50 µm (left), 20 µm (right). g Statistics of the percentage of engrafted DsRed⁺ cells with Annexin V⁺ in injected pig blastocysts; mean + SEM of 16 (UH10-DsRed) or 12 (UH10-DsRed + BMI1) samples, ***p < 0.001. h Statistics of the number of DsRed⁺ cells injected in pig blastocysts; mean ± SEM of 16 (UH10-DsRed) or 12 (UH10-DsRed + BMI1) samples, **p < 0.01. i Representative images of pig blastocyst injected with UH10-DsRed + BMI1 stained with antibodies against the indicated markers. Two indicated DsRed⁺ cells were injected to 8C stage pig embryos. The integrated UH10-DsRed⁺ with BMI1 expression coexpress OCT4 or CDX2 in cultured chimeric embryos after additional 4 days’ culture in vitro. White arrow, DsRed⁺/OCT4⁺ cells; yellow arrow, DsRed⁺/CDX2⁺ cells. Scale bars, 20 µm. j Summary of chimera assays injected with the indicated DsRed⁺ cells at the 8C stage pig embryo, and the injected embryos were cultured for additional 4 days to blastocysts