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. 2018 Nov 7;8:16510. doi: 10.1038/s41598-018-34503-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Viral titer and genome copies in one-step growth kinetics. Confluent DEF cells monolayers were infected with each virus shown at an MOI of 2. Viral titer and viral copies of infected cells were determined at the indicated time points by measuring TCID50 on DEF cells. All titrations were carried out in three independent experiment. The titers and copies obtained were averaged, and the standard error of the mean was calculated each time point. (A) Viral copies. (B) Viral titer. (C) Viral copies and titer. (D) Genome/TCID₅₀ ratios.