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. 2018 Nov 7;8:16467. doi: 10.1038/s41598-018-34416-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of pH on AprA and AprA-PPC activity and stability with or without Ca²⁺. (A) The optimal pH for AprA activity (filled square) and AprA-PPC activity (empty square) without Ca²⁺; (B) pH stability of AprA with (filled triangle) or without (empty triangle) Ca²⁺; (C) pH stability of AprA-PPC with (filled circle) or without (empty circle) Ca²⁺. The measurements were performed in three independent experiments. Error bars represent standard deviations.