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. 2018 Nov 7;8:16467. doi: 10.1038/s41598-018-34416-5

Table 4.

Substrate specificity and kinetic parameters of recombinant AprA and AprA-PPC.

Substrate	AprA			AprA-PPC
Substrate	Relative activity (%)	K_m (mg ml⁻¹)	V_max (U mg⁻¹)	Relative activity (%)	K_m (mg ml⁻¹)	V_max (U mg⁻¹)
Casein	100.0	3.76 ± 0.08	57538.5 ± 785.4	100.0	3.98 ± 0.10	108722.1 ± 1801.2
BSA	17.3 ± 0.3	8.89 ± 0.21	11198.4 ± 223.3	21.7 ± 0.4	9.06 ± 0.24	25147.1 ± 348.1
Gelatin	8.0 ± 0.1	10.41 ± 0.29	5932.5 ± 97.5	8.4 ± 0.2	10.59 ± 0.31	9873.2 ± 131.6
Keratin	51.3 ± 1.2	5.38 ± 0.16	27921.1 ± 357.1	51.7 ± 1.4	5.25 ± 0.19	53272.6 ± 628.7
Skim milk	47.2 ± 1.0	6.02 ± 0.20	24378.3 ± 322.5	46.2 ± 1.1	6.12 ± 0.20	45196.8 ± 512.4
Collagen	2.5 ± 0.1	ND^a	ND	2.6 ± 0.2	ND	ND

^aND means not determined.