Table 2. Unique Histone Proteoforms Identified Using ProSight Absolute Mass Search against the Human Proteomea.
| LC-MS method | C < 3 | 3 ≤ C ≤ 45 | C > 45 | Total Unique IDs with C > 3 (total IDs) |
|---|---|---|---|---|
| 1DLC RPLC–UVPD-MS | 147 | 51 | 16 | 67 (155) |
| 1DLC WCX-HILIC–UVPD-MS | 108 | 29 | 8 | 37 (128) |
| 2DLC WCX-HILIC/a×m/RPLC-UVPD-MS R1 | 158 | 61 | 28 | 87 (173) |
| 2DLC WCX-HILIC/a×m/RPLC-UVPD-MS R2 | 175 | 62 | 30 | 92 (189) |
| 2DLCWCX-HILIC/a×m/RPLC-UVPD-MS R3 | 173 | 61 | 31 | 92 (190) |
a
Identifications are sorted according to their C score. The total number of identified proteoforms is given in parentheses. Data filtered for P < 1E–4. Protein IDs available in Table S4 of the Supporting Information.