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. 2018 Oct 31;12:96. doi: 10.3389/fncir.2018.00096

FIGURE 7.

FIGURE 7

Rac1 cKO brain slices exhibited altered oscillatory activities. The rate of power (%) (see Materials and Methods) which represents the percentage of the signal power spectrum (mV2/Hz) of each oscillation in relation to the power spectrum of whole signal (mV2/Hz). The following graphs show the ratio of power that exists in the specific range for each oscillation [Delta (1–4 Hz), Theta (4–8 Hz), Alpha (8–12 Hz) and Beta (12–30 Hz) and Total Gamma (30–150 Hz)]. The rate of power was calculated in three conditions, control aCSF, high K+ aCSF and high K+ aCSF plus 2 μM diazepam, for each genotype. (A) Bar graph showing the rate of power of each frequency domain in heterozygous (left) and Rac1 cKO (right) brain slices in control aCSF, revealing that the most dominant oscillatory activity was the gamma (30–150 Hz) frequency domain in the heterozygous and Rac1 cKO acute brain slices across all conditions. [n = 5 slices from five Rac1 cKO mice; n = 5 slices from five heterozygous mice, heterozygous: one-way ANOVA, F(1,10) = 3.067, p = 0.01 and Rac1 cKO: ordinary one-way ANOVA, F(1,10) = 18.06, p = 0.01, comparison between groups and within groups with Tukey post hoc test]. (B) Bar graph showing the rate of power in delta (1–4 Hz) frequency domain. A trend for increase in high K+ aCSF and in K+ aCSF plus diazepam was observed in heterozygous brain slices. In Rac1 cKO brain slices, the rate of delta power was lower compared to heterozygous brain slices and unaltered among all three conditions [n = 5 slices from five Rac1 cKO mice; n = 5 slices from five heterozygous mice, one-way ANOVA, F(1,15) = 1.239, p = 0.01, comparison between groups and within groups with Tukey post hoc test]. (C) Bar graph showing the rate of power in theta (4–7 Hz) frequency domain. A trend for increase in high K+ aCSF and in K+ aCSF plus diazepam was observed in heterozygous brain slices. In Rac1 cKO brain slices, the rate of theta power was lower compared to heterozygous brain slices and unaltered among all three conditions [n = 5 slices from five Rac1 cKO mice; n = 5 slices from five heterozygous mice, ordinary one-way ANOVA, F(1,15) = 0.7201, p = 0.01, comparison between groups and within groups with Tukey post hoc test]. (D) Bar graph showing the rate of power in alpha (8–12 Hz) frequency domain. A trend for increase in high K+ aCSF and in K+ aCSF plus diazepam in heterozygous brain slices. In Rac1 cKO brain slices, the rate of theta power was lower compared to heterozygous brain slices and unaltered among all three conditions [n = 5 slices from five Rac1 cKO mice; n = 5 slices from five heterozygous mice, ordinary one-way ANOVA, F(1,15) = 0.6602, p = 0.01, comparison between groups and within groups with Tukey post hoc test]. (E) Bar graph showing the rate of power in beta (13–30 Hz) frequency domain was lower in heterozygous than Rac1 cKO brain slices. In either genotype, the rate of power did not change in any of the three conditions [n = 5 slices from five Rac1 cKO mice; n = 5 slices from five heterozygous mice, ordinary one-way ANOVA, F(1,15) = 3.778, p = 0.01, comparison between groups and within groups with Tukey post hoc test]. (F) Bar graph showing the rate of power in total gamma (30–150 Hz) frequency domain. A trend for increase was observed in high K+ aCSF plus diazepam in heterozygous brain slices, compared to high K+ or control aCSF. In Rac1 cKO brain slices the rate, of gamma power was lower compared to heterozygous brain slices and was unaltered in all three [n = 5 slices from five Rac1 cKO mice; n = 5 slices from five heterozygous mice, ordinary one-way ANOVA, F(1,15) = 0.0478, p = 0.01, comparison between groups and within groups with Tukey post hoc test].