Fig. 5.

Ibuprofen blocked LPS induced oxidative stress in both wild-type and Atm^−/− mice. a DNA oxidation was identified by 8-oxo-dG immunohistochemistry. The nuclear and cytoplasmic intensity of the 8-oxo-dG signal was quantified separately b Confocal images of neurons demonstrating that the nuclear and cytoplasmic signal of 8-oxo-dG invidiously. The second type of 8-oxo cellular distribution pattern was shown in the lower panel with a clean cut of the nuclear region. c Relative total intensity measured by ImageJ. d Relative nuclear intensity measured by ImageJ. e Percentage of 8-oxo positive neurons, which includes both types of 8-oxo-dG patterns in Fig. 5b, in MAP2 and NeuN double-positive cells (data not shown). Scale bar = 10 μm. n = 3–4 for each group. Two-way ANOVA was used for analyzing differences in the effects of LPS injection and ibuprofen within the same genotype. * p < 0.05 as indicated in the graphs