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. 2018 Nov 6;18:269. doi: 10.1186/s12870-018-1471-6

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Adaptor ligation efficiency. Electrophoretic detection (a) and analysis of band intensity (b). M: 100 bp marker Lane 1: HaeIII adapter 1 primer 1/204F; Lane 2: HaeIII adapter 1 primer 1/204R; Lane 3: HaeIII adapter 2R primer 1/204F; Lane 4: HaeIII adapter 2R primer 1/204R; Lane 5: AluI adapter 1 primer 1/204F; Lane 6: AluI adapter 1 primer 1/204R; Lane 7: AluI adapter 2R primer 1/204F; Lane 8: AluI adapter 2R primer 1/204R; Lane 9: RsaI adapter 1 primer 1/204F; Lane 10: RsaI adapter 1 primer 1/204R; Lane 11: RsaI adapter 2R primer 1/204F; Lane 12: RsaI adapter 2R primer 1/204R