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. 2018 Nov 6;18:269. doi: 10.1186/s12870-018-1471-6

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — PCR analysis of adapter ligation efficiency analysis. Electrophoretic detection (a) and analysis of band intensity (b). M: 100 bp marker; Lane 1: 150 ng/μL experimental sample; Lane 2: 150 ng/μL control tester sample. Dashed line indicates 25% of control tester sample intensity