Fig. 4.

Electrophoresis of suppression PCR products after SSH. M: 100 bp marker; Lane 1: subtract first PCR 62 °C; Lane 2: unsubtract first PCR 62 °C; Lane 3: subtract first PCR 64 °C; Lane 4: unsubtracted first PCR 64 °C; Lane 5: subtracted first PCR 66 °C; Lane 6: unsubtracted first PCR 66 °C; Lane 7: subtracted first PCR 68 °C; Lane 8: unsubtracted first PCR 68 °C; Lane 9: subtracted second PCR 62 °C; Lane 10: unsubtracted second PCR 62 °C; Lane 11: subtracted second PCR 64 °C; Lane 12: unsubtracted second PCR 64 °C; Lane 13: subtracted second PCR 66 °C; Lane 14: unsubtracted second PCR 66 °C; Lane 15: subtracted second PCR 68 °C; Lane 16: unsubtracted second PCR 68 °C