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. 2018 Oct 29;9:2477. doi: 10.3389/fimmu.2018.02477

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Copyright © 2018 Puente-Marin, Nombela, Chico, Ciordia, Mena, Coll, Mercado and Ortega-Villaizan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immune proteins expression in GVHSV-transfected RBCs. (A) Immune protein expression measured by flow cytometry and calculated by the formula MRFI (mean relative fluorescence intensity) = fluorescence in pmTFP1GVHSV- transfected RBCs MRFI (mean relative fluorescence intensity) = fluorescence in pmTFP1GVHSV- transfected RBCs /fluorescence in pmTFP1-transfected RBCs. (B) Representative immunofluorescence of pmTFP1- and pmTFP1GVHSV-transfected RBCs (protein [APC, red] and nuclei [DAPI, blue]). Fluorescence images were taken with 60 × magnification.