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. Author manuscript; available in PMC: 2018 Dec 27.
Published in final edited form as: Nature. 2018 Jun 27;559(7712):67–72. doi: 10.1038/s41586-018-0255-3

Figure 3. Flumazenil interactions at the benzodiazepine binding site.

Figure 3

a, Electrophysiology of the cryo-EM construct showing flumazenil (3 μM) blocks GABA-A receptor potentiation by diazepam (1 μM). n=3 independent experiments. b, Flumazenil and diazepam chemical structures. c, View from synapse, as in Fig. 2; box indicates flumazenil bound at α1-γ2 interface. d, Schematic of flumazenil interactions showing electrostatic (dashes) and hydrophobic interactions (eyelashes). e,f, Detailed architecture of flumazenil binding pocket boxed in c, with orientations and representations as in Fig. 2e-f.