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. 2018 Oct 18;2018:7156150. doi: 10.1155/2018/7156150

Table 1.

RT-qPCR primer sequences and binding temperatures (Temp).

GENE SEQUENCE Fwd (5→3) SEQUENCE Rev (5→3) Temp
HIF-1 α ACGTGTTATCTGTCGCTTTGAG ATCGTCTGGCTGCTGTAATAATG 59°C

EGLN3 GATGCTGAAGAAAGGGC CTGGCAAAGAGAGTATCTG 60°C

TGF- β 1 GCGTGCTAATGGTGGAAAC CGGAGCTCTTGATGTGTTGAAGA 60°C

VEGF ATGACGAGGGCCTGGAGTGTG CCTATGTGCTGGCCTTGGTGAG 60°C

eNOS AAG AGG AAG GAG TCC AGT AAC ACA GA ACG AGC AAA GGC GCA GAA 60°C

Tie-2 TGCCCAGATATTGGTGTCCT CTCATAAAGCGTGGTATTCACGTA 60°C

GAPDH ATGACGAGGGCCTGGAGTGTG CCTATGTGCTGGCCTTGGTGAG 60°C