Table 1.

Modified neurological deficit scores.

Items	Scores
Neurological deficit scoresa	0-4
Tail testb	0-2
Placing test	0-7
Visionc	0-1
Tactile sensationd	0-6
Forelimbs	0-2
Hindlimbs	0-2
Lateral	0-2
Total	0-13

a: scores were as follows: 0 points, no neurological deficit; 1 point, left forelimb not fully extended; 2 points, circling to the left; 3 points, dumping to the left; 4 points, no spontaneous activity or coma.

b: scores were as follows: 0 points, no flexion; 1 point, the left forelimb flexion or trunk torsion to the right side; 2 points, the left forelimb flexion and trunk torsion to the right side

c: scores were as follows: the tester held the rat face close to the edge of the table, whisker cut short. Normally, rats would reach the desktop by their bilateral forelimbs. 0 points, the rats showed normal; 1 point, left forelimb could not reach the table.

d: scores were as follows: rats were placed on the edge of the table, with forelimbs hanging at the edge of the table and free move. One side of forelimbs were gently pulled down. The tester observed the forelimbs back to the original place and repeated the other side of the forelimbs, using the same method to observe the activities of hindlimbs. Finally, the rats were placed in parallel to the table edge, and the lateral movement of the forelimbs was observed. 0 points, normal stretch (forward, backward, or lateral); 1 point, stretch but more than 2s delay and/or extension not complete; 2 points, no stretch.