Figure 5.

Neuritin increases I _Ca amplitude without altering voltage‐gating properties of Ca²⁺ channels. (a) Representative current traces obtained from control and neuritin‐treated cerebellar granule neurons (CGNs). I _Ca was elicited by depolarization from a holding potential of −80 mV to 10 mV for 250 ms. (b) Quantification of I _Ca amplitude recorded from control and neuritin‐treated CGNs cultured for different lengths of time (3 days Ctl, n = 48; 3 days Nrn, n = 31; 5 days Ctl, n = 48; 5 days Nrn, n = 37; 7 days Ctl, n = 36; 7 days Nrn, n = 21). (c) Representative traces obtained from control and neuritin‐treated CGNs with a steady‐state voltage protocol. (d) Voltage‐dependent activation curve of I _Ca in CGNs cultured in the absence or presence of neuritin (Ctl, n = 43; Nrn, n = 36). (e) Plot of normalized conductance as a function of command potential in CGNs cultured in the absence or presence of neuritin (Ctl, n = 21; Nrn, n = 28). Data points were fitted with a sigmoidal function using the Boltzmann model. (f) Quantitative analysis of the effects of neuritin on membrane capacitance (Ctl, n = 48; Nrn, n = 37). Data represent mean ± SEM, n indicates the number of cells. *p < 0.05, **p < 0.01, ***p < 0.001 between two groups connected with a straight line (unpaired t test or one‐way anova followed by Bonferroni post hoc test).