Figure 10.

Nuclear factor of activated T cells (NFAT)c4 silencing abrogates neuritin‐induced increases in cerebellar granule neuron (CGN) neurite length and spine density. (a) Micrographs showing the transfection efficiency of lentivirus expressing green fluorescent protein and a scrambled control sequence (Scramble) or siNFATc4. (b) Western blot and quantitative analyses of the suppression of NFATc4 expression by siNFATc4 (n = 3). (c and d) Representative images and quantification of the effects of neuritin on neurite length (c) (Scramble, n = 20; Scramble + Nrn, n = 20; siNFATc4, n = 22; siNFATc4 + Nrn, n = 17) and total spine density (d) (Scramble, n = 22; Scramble + Nrn, n = 19; siNFATc4, n = 19; siNFATc4 + Nrn, n = 19) in CGNs cultured in the absence or presence of siNFATc4. Results represent mean ± SEM, n indicates the number of experiments in western blot or number of cells in morphological analysis. ***p < 0.001 between two groups connected with a straight line (one‐way anova followed by Bonferroni post hoc test).