Table 2.

Kinetic parameters for demethylation of H1.4 peptides by recombinant human KDM4A (1 μm) measured using a formaldehyde dehydrogenase‐coupled demethylation assay 27. Data show the mean and standard deviation of three independent replicates. See Materials and methods for complete assay conditions

	K M/μm	k cat/s−1	k cat/K M
H3(1–15)K9me3	25.5 ± 4.9	0.31 ± 0.045	0.0124
H1.4(18–32)K26me3	32.3 ± 6.4	0.32 ± 0.049	0.0098
H3(29–43)K36me3	66.8 ± 5.4	0.12 ± 0.004	0.0019