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Validation of synthetic UFM1. A) SDS‐PAGE gel showing synthetic and expressed UFM1. B) CD measurements of expressed UFM1 and synthetic UFM1 made by linear synthesis or native chemical ligation. Synth. FL=UFM1 generated by linear synthesis; Synth. NCL=UFM1 generated by NCL (6). C) UBA5 reacts with both expressed and synthetic UFM1. The asterisk (*) indicates UFM1‐thioester‐UBA5.
